Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses
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چکیده
منابع مشابه
Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses.
Despite the considerable advantages that C. elegans offers for studying gene function in vivo, this system is quite challenging for in vivo electrophysiological analysis of channel function, particularly in neurons. A major problem is that C. elegans neurons are confined in a pressurized and hard-to-penetrate cuticle. Recently, a method for culturing C. elegans embryonic cells has been develope...
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Cell culture is an essential tool to study cell function. In C. elegans the ability to isolate and culture cells has been limited to embryonically derived cells. However, cells or blastomeres isolated from mixed stage embryos terminally differentiate within 24 hours of culture, thus precluding post-embryonic stage cell culture. We have developed an efficient and technically simple method for la...
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ژورنال
عنوان ژورنال: WormBook
سال: 2006
ISSN: 1551-8507
DOI: 10.1895/wormbook.1.122.1